CIRM: Research InternAt — CIRM: Scripps Research Institute
Must be enrolled in BIOT 498 and a participant in the CIRM: Bridges to Stem Cell Research Program.
In the Cline lab, we use human induced pluripotent stem cell (iPSC)-derived neurons and microglia with different APOE genotypes to investigate how APOE affects the function of these cell types.
Our CIRM intern will contribute to our studies on Alzheimer's disease by helping characterize the effect of APOE genotypes in iPSC-derived neurons using techniques like rt-qPCR to assess changes in gene expression, immunocytochemistry and confocal microscopy to assess the localization of synaptic proteins, as well as sparse neuron labeling to evaluate neuronal morphology and neurite structure. The intern will also be involved in studies to assess the response of neurons to microglia secreted signals.
The intern is mentored and closely guided by a graduate student and research assistant.
The intern will complete safety training required for all Scripps Research scientists.
Interview with members of the Cline lab at Scripps Research.
| Hours | Duration |
|---|---|
| 35 | hours per week |